Function of epithelial cells derived from the rests of Malassez: an in vitro investigation.

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Epithelial cell rests of Malassez (ERM) are quiescent epithelial remnants of the Hertwig’s epithelial root sheath (HERS) that are involved in the formation of tooth roots. ERM cells Cited by: 9. Cultured epithelial cells (E-cells) derived from the rests of Malassez exhibited a diploid karyotype with a modal number of 38 chromosomes, which is the number reported for the pig.

Epithelial cell rests of Malassez (ERM) are quiescent epithelial remnants of the Hertwig's epithelial root sheath (HERS) that are involved in the formation of tooth roots.

ERM cells are unique epithelial cells that remain in periodontal tissues throughout adult life. They have a functional role in the repair/regeneration of cement or by: 9. The epithelial cell rests of Malassez and the genesis of the dental cyst A.

Ten Cote, B.D.S., Ph.D.* Toronto, Ontario, Canada FACULTY OF DENTISTRY, UNIVERSITY OF TORONTO Activation of the epithelial cell rests of Malassez, both in. vivo and in vitro, involves a switch in their metabolism and the utilization of the by: M.J. Merrilees, J. Sodek, J.E.

Aubin, Effect of cells of epithelial rests of Malassez and endothelial cells on synthesis of glycosaminoglycans by periodontal ligament fibroblasts in vitro, Developmental Biology, /(83), 97, 1, (), ().Cited by: The epithelial cell rests of Malassez (ERM) are derived from Hertwig's epithelial root sheath (HERS).

During development the cells of HERS deposit a variety of molecules on the newly forming root. T. Yamanaka, A. Sakamoto, Y. Tanaka, et ion and serum-free culture of epithelial cells derived from epithelial rests of Malassez in human periodontal ligament In Vitro Cell Dev Biol Anim, 36 (), pp.

These HERS cells might undergo apoptosis or EMT to rest inside the periodontal ligament as epithelial cells rest of Malassez (ERM).

5 Animals like. The central core of epithelium disappears leaving only a double layer of basal epithelium known as Hertwig's epithelial root sheath (HERS). It directs root growth and gives rise to a fenestrated network of epithelial cells covering the root and is known as epithelial cell rests of Malassez (ERM).

Mitomycin C (MMC)-treated Hertwig’s epithelial root sheath/epithelial rests of Malassez (HERS/ERM) cells were used as a feeder layer for epithelial-like cell differentiation.

Two weeks after the seeding of human induced pluripotent stem cell (hiPSC) clumps on the feeder layer, differentiated epithelial-like cells were collected and characterized. To differentiate hESCs/hiPSCs into dental epithelial-like stem cells, this study investigated the hypothesis that direct interactions between pluripotent stem cells, such as hESCs or hiPSCs, and Hertwig’s epithelial root sheath/epithelial rests of Malassez (HERS/ERM) cell line may induce epithelial differentiation.

In this study, novel human-derived epithelial-like cells (hEPLCs) lines were established from periodontal ligament (PDL) tissues, which were composed of a variety of cell types and exhibited complex cellular activities. To elucidate the putative features distinguishing these from epithelial rest of Malassez (ERM), we characterized hEPLCs based on cell lineage markers and tight junction protein.

Objectives. The purpose of this study was to evaluate the synergistic effect of epithelial rests of Malassez cells (ERM) and transforming growth factor-β 1 (TGF-β 1) on proliferation, cementogenic and osteogenic differentiation of stem cells derived. The epithelial cell rests of Malassez (ERM) are odontogenic epithelial cells located within the periodontal ligament matrix.

While their function is unknown, they may support tissue homeostasis and maintain periodontal ligament space or even contribute to periodontal regeneration.

Stem/progenitor cells are undifferentiated cells characterized by their exclusive ability for self-renewal and multilineage differentiation potential.

In recent years, researchers and investigations explored the prospect of employing stem/progenitor cell therapy in regenerative medicine, especially stem/progenitor cells originating from the oral tissues.

In this context, the regeneration of. Kenichi Matsuzaka, Eitoyo Kokubu, Takashi Inoue, The effects of epithelial rests of Malassez cells on periodontal ligament fibroblasts against centrifugal forces in vitro, Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology, /, 25, 2, (), ().

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We report a novel method for the isolation of adult human epithelial stem cells (hEpiSCs) from the epithelial component of the periodontal ligament—the human epithelial cell rests of Malassez (hERM).

hEpiSC-rich integrin-α6 +ve hERM cells derived by fluorometry can be clonally expanded, can grow organoids, and express the markers of pluripotency (OCT4, NANOG, SOX2), polycomb protein.

Introduction. M esenchymal stem cells (MSC) have received significant attention in recent years with regard to their suitability for use in cellular therapies due to their high growth potential and multipotential differentiation capacity. MSC were first identified in the bone marrow but have since then been derived from a wide range of tissues, including, but not limited to, bone marrow.

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In vitro cultural parameters and protein and prostaglandin secretion of epithelial cells derived from porcine rests of Malassez. Arch Oral Biol. ;24(3) Carpenter G. Brunette DM, Heersche JN, Purdon AD, Sodek J, Moe HK, Assuras JN: In-vitro cultural parameters and protein and prostaglandin secretion of epithelial cells derived from porcine rests of Malassez.

Arch Oral Biol./(79) CAS. Ji‐Hyun Lee, Dong‐Seol Lee, Hyun Nam, Gene Lee, Byoung‐Moo Seo, Young‐Sik Cho, Hyun‐Sook Bae, Joo‐Cheol Park, Dental follicle cells and cementoblasts induce apoptosis of ameloblast‐lineage and Hertwig’s epithelial root sheath/epithelial rests of Malassez cells through the Fas–Fas ligand pathway, European Journal of Oral.

Hertwig’s epithelial root sheath (HERS) is important in guiding tooth root formation by differentiating into cementoblasts through epithelial–mesenchymal transition (EMT) and inducing odontoblastic differentiation of dental papilla through epithelial–mesenchymal interaction (EMI) during the tooth root development.

Thus, HERS cells are critical for cementum and dentin formation and. Stem cells are capable of renewing themselves through cell division and have the remarkable ability to differentiate into many different types of cells.

They therefore have the potential to become a central tool in regenerative medicine. During the last decade, advances in tissue engineering and stem cell-based tooth regeneration have provided realistic and attractive means of replacing lost.

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Grachtchouk et al showed that disrupting normal Hh signaling at the epithelial cell rests of Malassez (ERM) induced KCOTs.

Gao et al induced KCOTs from ERM by disrupting TGF-β signaling. ERM are odontogenic epithelial cells in the periodontal ligaments and are normally quiescent and predicted to work during periodontal regeneration (28,29).

Dental epithelial stem cells (DESCs), which were isolated from the developing third molar or epithelial rests of Malassez, were also studied for tooth and periodontal regeneration (Harada et al., ; Nam et al., ) and can generate enamel–dentin‐like complex structures in vivo after being combined with dental mesenchymal cells (Honda.

E11 immunostaining included cementoblasts and cementocytes of the cellular cementum, epithelial rests of Malassez, osteoblasts, and peripheral osteocytes in alveolar bone, and, in addition, basal cells of the gingival epithelium and endothelial cells. Morphological description of the repair process Experimental days 0–7.

Lung mitochondria preferentially use glucose-derived substrates, such as pyruvate, for oxidative energy production; however, other energy sources, including FAs, Krebs cycle intermediates, glycerolphosphate, and glutamate, are also used, with the highest O 2 consumption rates achieved with succinate as a substrate ().Lung mitochondria also have a unique advantageous metabolic.

Based on this model, a population of long-lived, label-retaining cells residing in the OEE and the adjacent SR region in the proximal part of the adult mouse laCL were proposed to function as dental epithelial stem cells, (Fig.

5A) (Harada et al., ; Biehs et al., ). Genetic lineage tracing – one of the principal methods for. Apart from the cytoprotective functions of K14, very little is known about K14 regulatory functions, since the K14 knockout mice show postnatal lethality.

In this study, K14 expression was inhibited using RNA interference in cell lines derived from stratified epithelia to study the K14 functions in epithelial homeostasis. [2,3], PDL stem cells (PDLSCs), epithelial cell rests of Malassez that are descendants of the Hertwig’s epithelial root sheath (HERS), endothelial cells, and others.

Especially, PDLSCs have the potentials to differentiate into PDL fibroblasts, cementoblasts and osteoblasts []. Aged. Role of the Epithelial Cell Rests of Malassez in Periodontal Homeostasis and Regeneration - A Review Current Stem Cell Research & Therapy; Pivotal Roles of Interleukin as the Epicenter of Bone Loss Diseases Current Pharmaceutical Design.

An ultrastructural evaluation of the relationship between epithelial rests of Malassez and orthodontic root resorption and repair in man.

Origins and functions of cells essential for periodontal repair: the role of fibroblasts in tissue homeostasis. Human dentin matrix induces cartilage formation in vitro by mesenchymal cells derived.Stem Cell Biology and Tissue Engineering in Dental Sciences bridges the gap left by many tissue engineering and stem cell biology titles to highlight the significance of translational research in this field in the medical sciences.

It compiles basic developmental biology with keen focus on cell and matrix biology, stem cells with relevance to tissue engineering biomaterials including.